Modified Simple Colorimetry of Dehydrogenase Activity in Shellfishes using Tetrazolium Chloride
نویسندگان
چکیده
منابع مشابه
detection of volatile compounds of medicinal plants with some nano-sorbents using modified or new methodologies and investigation of antioxidant activity of their methanolic extracts
in this work, a novel and fast method for direct analysis of volatile compounds (davc) of medicinal plants has been developed by holding a filament from different parts of a plant in the gc injection port. the extraction and analysis of volatile components of a small amount of plant were carried out in one-step without any sample preparation. after optimization of temperature, extraction time a...
Colorimetry of diaphorase in commercial preparations and clinical chemical reagents by use of tetrazolium salts.
We describe a procedure for assay of diaphorase activity in commercial purified preparations and in clinical chemical reagents by use of iodonitrotetrazolium chloride or other tetrazolium salts. The method is based on measurement of the formazan produced by enzymic reduction of tetrazolium salts in the presence of NADH. The assay procedure has been optimized for linear kinetics, simplicity of o...
متن کاملcomparison of catalytic activity of heteropoly compounds in the synthesis of bis(indolyl)alkanes.
heteropoly acids (hpa) and their salts have advantages as catalysts which make them both economically and environmentally attractive, strong br?nsted acidity, exhibiting fast reversible multi-electron redox transformations under rather mild conditions, very high solubility in polar solvents, fairly high thermal stability in the solid states, and efficient oxidizing ability, so that they are imp...
15 صفحه اولSuccinate Dehydrogenase Activity Assay in situ with Blue Tetrazolium Salt in Crabtree-Positive Saccharomyces cerevisiae Strain
A spectrophotometric method for determining succinate dehydrogenase (SDH) activity assay in azide-sensitive yeast Saccharomyces cerevisiae has been developed. The permeabilization of yeast cells by 0.05 % digitonin permitted to study yeast enzymatic activity in situ. The reduction of blue tetrazolium salt (BT) to blue tetrazolium formazan (BTf) was conducted in the presence of phenazine methosu...
متن کاملDetection of rough dissociants of Pasteurella pestis with tetrazolium chloride.
Lederberg (J. Bact., 56, 695, 1948) reported the use of triphenyl tetrazolium chloride to detect fermentative variants of Ewherichia coli K-12. We have found that smooth and rough colonies of Pasteurella pestis could be identified easily when grown on tryptose (Difco) agar containing this compound. On many routine and modified laboratory media that did not contain tetrazolium, differentiation w...
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ژورنال
عنوان ژورنال: Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
سال: 1986
ISSN: 0015-6426,1882-1006
DOI: 10.3358/shokueishi.27.487